Oncoprot

A combined laser microdissection and mass spectrometry method for proteomic analysis

Oncoprot image 2

Description

The Oncoprot platform is affiliated to the BaRITOn unit and belongs to the TBMCore (INSERM US005). Oncoprot is also certified by SIRIC BRIO.

A combined laser microdissection and mass spectrometry method for proteomic analysis of Formalin-fixed, Paraffin-embedded (FFPE) tissue sections. Mass spectrometry is the method of choice to identify, characterize and quantify proteins in a complex sample. This procedure can be applied to classical FFPE tumor sections and compatible to biopsies.

 

Activities

  • To identify signature biomarkers in tumors
  • To compare the levels of protein expression between tumoral and non tumoral tissue derived from the same patient
  • To discriminate patients good and bad responders to chemotherapies.
  • To develop targeted quantification by mass spectrometry of a set of choice of known biomarkers.

 

Infrastructure and Equipment

  • LEICA RM2255 automatic microtome of Histopathology plateform
  • PALM type 4 automated laser microdissector (Zeiss) on the laser microdissection platform of the Magendie Neurocenter (INSERM U 862)
  • Q-Exactive mass spectrometer (Thermo) of the Proteome Plateform of the Functional Genomic Center of Bordeaux

 

People

Scientific manager :
Frédéric Saltel
frederic.saltel@inserm.fr

Project manager :
Anne-Aurélie Raymond
anne-aurelie.raymond@inserm.fr

Research and development engineer :
Sylvaine Di Tommaso
s.di-tommaso@ast-innovations.com

 Tel : +33 (0)5 57 57 17 07

Our Partners

logo protéomique

logo CBIB

logo Magendie

logo TransBioMed

logo SATT

logo BIC

Logo SIRIC BRIO

Updated on 30/06/2017

Contacts

Frédéric SALTEL
Scientific Manager

+33 (0)5 57 57 17 07
Contact by email

Anne-Aurélie RAYMOND
Project Manager

Oncoprot

News

Oncoprot at Meet2Win 2017 Interview of Anne-Aurélie Raymond: https://vimeo.com/222167130