A combined laser microdissection and mass spectrometry method for proteomic analysis
A combined laser microdissection and mass spectrometry method for proteomic analysis of Formalin-fixed, Paraffin-embedded (FFPE) tissue sections. Mass spectrometry is the method of choice to identify, characterize and quantify proteins in a complex sample. This procedure can be applied to classical FFPE tumor sections and compatible to biopsies.
- To identify signature biomarkers in tumors
- To compare the levels of protein expression between tumoral and non tumoral tissue derived from the same patient
- To discriminate patients good and bad responders to chemotherapies.
- To develop targeted quantification by mass spectrometry of a set of choice of known biomarkers.
Infrastructure and Equipment
- LEICA RM2255 automatic microtome of Histopathology plateform
- PALM type 4 automated laser microdissector (Zeiss) on the laser microdissection platform of the Magendie Neurocenter (INSERM U 862)
- Q-Exactive mass spectrometer (Thermo) of the Proteome Plateform of the Functional Genomic Center of Bordeaux
Scientific manager :
Project manager :
Research and development engineer :
Sylvaine Di Tommaso
Tel : +33 (0)5 57 57 17 07
+33 (0)5 57 57 17 07
Contact by email
Oncoprot at Meet2Win 2017 Interview of Anne-Aurélie Raymond: https://vimeo.com/222167130