Oncoprot

A combined laser microdissection and mass spectrometry method for proteomic analysis

Description

The Oncoprot platform is affiliated to the BaRITOn unit and belongs to the TBMCore (INSERM US005). Oncoprot is also certified by SIRIC BRIO.

A combined laser microdissection and mass spectrometry method for proteomic analysis of Formalin-fixed, Paraffin-embedded (FFPE) tissue sections. Mass spectrometry is the method of choice to identify, characterize and quantify proteins in a complex sample. This procedure can be applied to classical FFPE tumor sections and compatible to biopsies.

Activities

To identify signature biomarkers in tumors
To compare the levels of protein expression between tumoral and non tumoral tissue derived from the same patient
To discriminate patients good and bad responders to chemotherapies.
To develop targeted quantification by mass spectrometry of a set of choice of known biomarkers.

Infrastructure and Equipment

LEICA RM2255 automatic microtome of Histopathology plateform
PALM type 4 automated laser microdissector (Zeiss) on the laser microdissection platform of the Magendie Neurocenter (INSERM U 862)
Q-Exactive mass spectrometer (Thermo) of the Proteome Plateform of the Functional Genomic Center of Bordeaux